T9028
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 | Immunohistochemistry Staining of Sertoli cell microtubules within a Stage VII seminiferous tubule of an adult male rat using Monoclonal Anti-Tyrosine Tubulin, Clone No. TUB-1A2 (Cat. No. T9028). The heavy staining surrounding the Sertoli cell nuclei indicates a collapse of the vimentin intermediate filament network following treatment. Interstitial cells are also stained. From J. Wenz and M. Nakai, Univ. of Illinois, College of Med., Dept. of Veterinary Biosciences, Urbana, IL. |
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T9028
Sigma
Monoclonal Anti-Tubulin, Tyrosine antibody produced in mouse
clone TUB-1A2, ascites fluid
| MDL number: | MFCD00164753 |
Description
| Immunogen | peptide containing the carboxy-terminal amino acids of α-tubulin |
| Specificity | The antibody reacts against tubulin's C-terminal tyrosine in immunoblotting assays and may be used for localization of this epitope in cultured cells or tissue sections. |
Properties
| antibody form | ascites fluid |
| clone | TUB-1A2 (monoclonal) |
| contains | 15 mM sodium azide |
| species reactivity | plant, animal, human |
| application(s) | immunoblotting: suitable |
| indirect immunofluorescence: 1:800 using cultured chicken fibroblasts | |
| microarray: suitable | |
| isotype | IgG3 |
| Gene Information | human ... TUBA4A(7277), TUBB(203068) |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
| WGK Germany | 3 |
Related Products
| Array | XP725, Panorama® Antibody Microarray - XPRESS Profiler725 Kit |
References
| reference | Kreis, T.E. EMBO J. 6, 2597, (1987) Abstract |
| Kojima, H., et al., Mechanical Properties of Inner-Arm Dynein-F (Dynein I1) Studied With in vitro Motility Assays Biophys. J. 93, 886-894, (2007) Abstract | |
| Uren, A., et al., Wnt-3a and Dickkopf-1 stimulate neurite outgrowth in Ewing tumor cells via a Frizzled 3 and c-Jun N-terminal kinase-dependent mechanism. Mol. Cell. Biol. 28, 2368-2379, (2008) Abstract | |
| Shiels, B.R., et al., A Theileria annulata DNA Binding Protein Localized to the Host Cell Nucleus Alters the Phenotype of a Bovine Macrophage Cell Line Eukaryot. Cell 3, 495-505, (2004) Abstract |
